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Image Search Results
Journal: STAR Protocols
Article Title: Protocol for Efficient CRISPR/Cas9/AAV-Mediated Homologous Recombination in Mouse Hematopoietic Stem and Progenitor Cells
doi: 10.1016/j.xpro.2020.100028
Figure Lengend Snippet: FACS Plots Showing Lin − Sca1 + c-Kit + (LSK) Cell Fractions after MACS Purification and 2 Days of Culture Bone marrow cells were stained with α-mouse lineage cocktail, Sca1, and c-Kit antibodies. (A) The negative fraction of the LS column should be almost completely depleted of Sca1 + cells. (B) The positive fraction eluted from the LS or MS separation column is enriched in LSK cells and progenitor cells, which all express Sca1. The LSK population should comprise 6 to 8% of the Sca1 + cell fraction. Lymphoid progenitor cells require IL-7 to survive and will be depleted upon culturing with 50 ng/mL mTPO, mSCF, mFLT-3, and hIL-11. (C) Purified LSK cell fraction after 2 days of culture in HSPC medium.
Article Snippet:
Techniques: Purification, Staining
Journal: STAR Protocols
Article Title: Protocol for Efficient CRISPR/Cas9/AAV-Mediated Homologous Recombination in Mouse Hematopoietic Stem and Progenitor Cells
doi: 10.1016/j.xpro.2020.100028
Figure Lengend Snippet:
Article Snippet:
Techniques: Virus, Recombinant, DNA Extraction, Cloning, Modification, Plasmid Preparation, Software, Adhesive, Real-time Polymerase Chain Reaction, Sterility, Centrifugation